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Image Search Results
Journal: Scientific Reports
Article Title: Inhaled sphingosine has no adverse side effects in isolated ventilated and perfused pig lungs
doi: 10.1038/s41598-021-97708-3
Figure Lengend Snippet: ( a , b ) Fluorescence intensity after staining with Cy3-coupled anti-sphingosine ( a ) and autofluorescence intensity without staining as control ( b ). Histological studies demonstrate an accumulation of sphingosine in bronchial epithelial cells after inhalation. EVLP minipig lungs were inhaled with sphingosine at concentrations of 125 µM sphingosine (SPH), 500 µM sphingosine (SPH) and with 0.125% octylglucopyranoside (OGP) as control. The lungs were subjected to bronchoscopy 30 and 150 min after the inhalation, biopsies were fixed in paraformaldehyde, embedded in paraffin and sectioned. Sections were stained with Cy3-coupled anti-sphingosine and without coupling (control). Shown are representative immune stainings. Given is the mean ± SD from 3 sections with 5 visual fields per animal (blinded tests), * p < 0.05, ** p < 0.01, *** p < 0.001, ANOVA.
Article Snippet:
Techniques: Fluorescence, Staining, Control